Aphid Eriosoma lonigerum and parasitoid Aphelinus mali.

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Dmitrii Musolin, 10.11.2013 22:49

Dear colleagues, good afternoon!

Someone from China wrote to me, looking for someone who could help with the material-aphids Eriosoma lonigerum and the parasitoid Aphelinus mali.

If you know someone who could help, please write (directly to the requester or to me).

Thank you in advance!

Good luck,

DM

---------- Forwarded message ----------
From: <hxzhouqd@sina.cn>
Date: 2013/11/6
Subject: Greetings from Hongxu Zhou, Qingdao
To: musolin <musolin@gmail.com>

Dear Dr Dmitrii L. Musolin,

I am writing to you and wish you could give me a favor. I would appreciate if you could do or recommend me some researchers working on apple tree pest management.

At present, we are studying the genetic variation of Aphelinus mali in China, and tend to make comparison with other countries. In 1950s Aphelinus mali were introduced from Russia to Qingdao to control woolly apple aphid. In order to make clear whether there is changes between Chinese and Russian population in genes. Thus we need some samples of woolly apple aphid (Eriosoma lonigerum Hausmann) and its parasite-Aphelinus mali in your country. It’s a hard work, and I really feel grateful if you could offer some help when you are convenient. If you could take some troubles to collect them for me, please see the attachment about the collecting method.
Best regards

Yours sincerely,
Hongxu Zhou
Qingdao Agricultural University

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The method for collecting woolly apple aphid:

At first, you will select one or several apple orchards damaged seriously by woolly apple aphid, and then cut several branches with much of woolly apple aphid in one apple tree, you would cut some branches from 15 apple trees, and branches cut from the same tree are put together. Bring them into laboratory, sweep the woolly apple aphid from each tree away onto a paper with a brash, and put woolly apple aphid into an eppendorf Centrifuge tube containing 95% ethanol. Among the things swept away from branches, there are black “pupa” parasitized by A. mali, put the black “pupa” in an empty eppendorf Centrifuge tube. After eclosion of A. mali through 5-10 days, put A. mali into another eppendorf Centrifuge tube containing 95% ethanol. That is to say, woolly apple aphid and Aphelinus mali collected from the same tree are put into different eppendorf Centrifuge tubes containing 95% ethanol respectively. Totally, there will be 15 tubes of woolly apple aphid and 15 tubes of Aphelinus mali. Please mark the tubes with numbers.

Finally, please take trouble to mail them to me. My address is: College of Agronomy and Plant Protection, Qingdao Agricultural University, number 700 Changcheng Road, Chengyang District, Qingdao, Shandong Province, China. Post number 266109