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Uniform illumination for the microscope

Community and ForumEntomological collectionsUniform illumination for the microscope

Arikain, 15.08.2013 12:44

Hello!
Can you tell me how it would be possible to achieve uniform illumination for a light microscope? I thought about removing the mirror altogether and putting a lamp on, but I haven't yet decided which one and how it could be done. Will a ring lamp be suitable, for example, and will the center not be darkened? The magnifications I work at mostly vary between 56x-400x. It is not very clear how it would be possible to adjust the lighting power.
I would like to achieve a uniform white illumination of micro-preparations.
I will be grateful for your help.

Comments

15.08.2013 13:53, Seneka

Hello!
Can you tell me how it would be possible to achieve uniform illumination for a light microscope? I thought about removing the mirror altogether and putting a lamp on, but I haven't yet decided which one and how it could be done. Will a ring lamp be suitable, for example, and will the center not be darkened? The magnifications I work at mostly vary between 56x-400x. It is not very clear how it would be possible to adjust the lighting power.
I would like to achieve a uniform white illumination of micro-preparations.
I will be grateful for your help.

Attach frosted white glass to the bottom of the slide table with tape or glue, and apply directional white light to the mirror, for example, from a diode flashlight with two brightness modes. Maybe it will be fine without glass.

15.08.2013 22:25, kovyl

Those. do you need to organize the illumination from the bottom? For the study of drugs in transmitted light?

15.08.2013 23:28, Arikain

Attach frosted white glass to the bottom of the slide table with tape or glue, and apply directional white light to the mirror, for example, from a diode flashlight with two brightness modes. Maybe it will be fine without glass.

Thank you so much for your reply!
I will try this option, such a glass is attached to the bottom of the condenser, it can be removed and left so, apparently you do not have to glue it.

15.08.2013 23:33, Arikain

Those. do you need to organize the illumination from the bottom? For the study of drugs in transmitted light?

Well, yes, the backlight is needed at the bottom for the study of drugs. I also photograph them, but the light is either uneven or a dim gray; I would like to achieve a uniform white and bright enough.

16.08.2013 13:01, Seneka

Well, yes, the backlight is needed at the bottom for the study of drugs. I also photograph them, but the light is either uneven or a dim gray; I would like to achieve a uniform white and bright enough.

Then just find a more powerful white light source, best of all on powerful white LEDs without light scattering lenses(they will only worsen the result). Get a more or less narrow white bunch of light, almost without chromatic distortion. The mirror will increase the beam density, so do not remove it.
It will be successful if the illuminator turns out to have several brightness modes. Otherwise, you will have to do the control scheme yourself on your knee or adjust the brightness by distance.
If you are completely smile.gifself-made, then take any old camera, disassemble the lens and pull out the aperture from there. The aperture has a handle or pin for manual adjustment of the aperture. Attach the diaphragm with glue, somewhere below the frosted glass. There are lenses with a built-in aperture, but this is on the other side of the drug.
Likes: 1

20.08.2013 20:33, Arikain

Thanks!
I did something like this, using a flashlight to direct the light into the mirror. It turns out really very well. The illumination can be adjusted with the camera, and it changes depending on the shutter speed set.

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