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Insect biology

Community and Forum → Other questions. Insects topics → Insect biology

Guestovitch, 02.01.2012 15:05

Fellow scientists!
I am looking for your advice,
when feeding double-stranded RNA to insects, will this dsRNA be detected in the hemolymph in its native state? Or will all dsRNA be splintered with RNases in the intestinal cells?
What do you think?

Comments

04.01.2012 18:12, Dergg

It is quite possible that the splinter will not even be in the cells of the intestine, but in its lumen. If a gene needs to be transduced into insect cells, it may be better to use an appropriate viral vector. To work with insects, baculovirus vectors are often used (they are, however, fungal).

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