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Immobilization and anesthesia

Community and Forum → Insects breeding → Immobilization and anesthesia

himik, 01.01.2015 6:00

Good afternoon!

I understand that January 1 is off-season for advice, but I still ask the entomological community for help.

We want to conduct a pilot study on cockroaches: Nauphoeta cinerea. We are currently developing a protocol. The test substances were decided to be administered by injection. The issue of immobilization and anesthesia became acute.

The research involves analyzing behavioral patterns that will naturally change under the stress of injections and manipulations. This is decided by a control group, in our case - a placebo.

But. We decided to carry out cold immobilization and anesthesia as the simplest and most affordable. However, in authoritative guidelines (Invertebrate Medicine, ed. G. A. Lewbart, 2012, p. 279), the following phrase is found: "Hypothermia (chilling/cooling)...should never be employed for surgical treatment or other procedures that may cause pain or be stressful" (hypothermia should never be used in the case of surgical interventions or other painful and stressful manipulations).

Is it so? Is hypothermia really not the best option for injecting?

If this is NOT the case, then what is the approximate recovery period, i.e., the time during which the insect has recovered after cooling and can be taken into the experiment? Is 1 hour enough? And half an hour?

If the cold doesn't suit you, what can you recommend? Given that the experiment itself takes 1 hour, and dozens of insects will participate in the study, I would like to use a method (accessible to the amateur), after which recovery occurs quickly.

Thank you.

Comments

01.01.2015 15:08, Vorona

There was an interesting topic here http://molbiol.ru/forums/index.php?showtop...hl=%E1%EE%EB%FC
As I recall, there are good reasons to doubt that insects feel pain....
(I'll sit next to you and listen. If you are interested, I can tell you about a small experience of studying the effect of drosophila staying in the freezer — I just had to immobilize them for laboratory work, preferably for a long time, or forever. A reversible immobilization of the same fruit flies by means of cold vladimirchi if you were interested, you can ask him about it)

02.01.2015 14:10, himik

Thank you, the topic is really interesting. :-)

02.01.2015 14:57, AGG

I don't remember who mol.gif shuffle.gifhas it, but there is a way with carbon dioxide. the work was carried out on the bumblebees? nests. we also gave a piece of marble to a graduate student for this case tongue.gifthe general meaning is this: acid is poured on a piece of solid wood=carbon dioxide is released=with a small exposure, everyone sleeps, and then runs like new. look, in net (it is possible, " a la Pesenko and companions of research.... nests....", it may be useful

This post was edited by AGG-02.01.2015 15: 07

02.01.2015 15:40, Vorona

There are even adaptations for this, but they are probably not cheap. If the chemist has not yet guessed to ask about the topic to which I implicitly referred, here it is — http://molbiol.ru/forums/index.php?showtopic=534665&hl=

02.01.2015 16:42, himik

2 AGG and Vorona.

Oh, I figured it out. Everything that could be downloaded on this occasion has already been downloaded. There were even thoughts of using nail polish removers without acetone (they contain ethyl acetate). Unfortunately, we can't afford to perform anesthesia with carbon dioxide. We are planning a pilot for 40 animals to try to reproduce the technique at minimal cost and see if the effects are detected. The amount we can spend on experiments is limited, unfortunately :-(

02.01.2015 18:32, AGG

for carbon dioxide, you need a bottle of vinegar essence (20-30 p) and break off a piece of marble wink.gifand when restoring steps and other monuments, and that is not necessary wink.gifas a result, your experiment may suffer only for the amount of an administrative fine (up to 5000r "for vandalism"), and then under the condition that the miner will tear off the slabs in broad daylight mausoleum or poor horseman-I don't know where you wink.gifare

03.01.2015 4:19, himik

I know what is necessary, I was taught this ;-). But. 1) along with carbon dioxide, essence vapors will also fly, 2) essence is not sold in stores in Belarus (only 6 %), 3) we have 40 animals. A maximum of 4 animals will be taken into the experiment on one day. This method is not rational, I can't get enough marble, and there is no Kipp machine.

But by downloading the book "William J. Bell (auth.)-The Laboratory Cockroach_ Experiments in cockroach anatomy, physiology and behavior-Springer Netherlands (1981)", I read that immobilization can be achieved by 5-minute immersion in warm water. o_O

This post was edited by himik-03.01.2015 04: 20

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