Stick insects are sick!

in one of the stick insects, the tips of the legs dry out =((

Poor devils! I'm holding Palochnikov myself, and I can't pass by without a care in the world. Something's probably wrong with their physiology. Try to diversify your diet. Spray every evening is not necessary, you can every other day. Excessive humidity can lead to the development of microflora, among which there is also a pathogenic one for insects, for example, the fungus Aspergyllus flavus. metallman. 92. Download the book on the maintenance and care of stick insects on the website http://zoometod.narod.ru/metod_bezpozv.html. There is a book called "spooks or stickmen"

metallman.92. tell me, can I buy offspring from you in the future?

if they survive, it's possible.... the fact is that I have only 4 of them, and everyone is sick, and two of them generally dry the tips of their paws and antennae

Oh, if you were in Chisinau, I would give you a dozen.

metallman. 92,I think your stickmen are just grown up,you can say old and therefore turned pale.By the way, pay attention to the litter,there are probably already eggs among it. And I think adults will soon die of old age.

what kind of adults are they?)) they only had 3-4 molts)))
eggs no

I know better,you took them from me, didn't you? 3-4 molts can not be such a thing!

then I won't understand anything=)))

BUTTERFLY, which of the stick insects are female?? "the thicker ones, or the thinner ones?"

Those who are older are females. The photo shows an obvious adult, which means that 3-4 molts were clearly not from birth. They are 8 - 10 centimeters long? Eggs should definitely be there! Blackening and drying of the limbs is a sign of old age in many insects. They don't have more than a month left to live, but then they should have some leftovers.Eggs are very small, look carefully! 2-3 mm in length

and how is drying regulated? hormonal? or is it micro-organisms? or what?

Drying is regulated by hormones, but can also be caused by microorganisms. Protozoa such as Microsporidia (the most famous are Nosema locustae, a parasite of locusts, and Nosema bombycis, a parasite of the silkworm (pebrina)) they can cause falling off of limbs, discoloration, in particular the appearance of spots, molting disorders. As a rule, they affect insects at the larval stage, if the imago survives, it is severely weakened, has the symptoms described above, and may also be infertile. Often, the pathogen is transmitted transovarially-through the egg from the mother to the offspring. True, I do not know microsporidia of the Annam stickworm. They are usually species-specific, and very rarely a single species parasitizes several hosts.

yes, the length without legs is about 10 cm.... well, we will search for eggs

why do they need hormones? and what's going on there? what drives away hemolymph?

As for hormonal regulation, my ideas are rather vague. I was more concerned with fungal and protozoal diseases of insects. However, the drying of the legs during the aging of the individual was observed in different species of other orthopteroids. In particular, the Moroccan and Asian locusts, Schistocercus, Italian prus, Madagascar cockroach, and the death's head cockroach.

fungal diseases?? I think I've hit the nail on the head...have you heard anything about the Labulbeniyevs? and about predatory ones..arthrobotris et al.?

We heard about them, but we didn't have to deal with them directly. Colleagues from Novosibirsk or Moscow offered to deal with predatory fungi-hyphomycetes, I don't remember exactly now, they wrote an international grant from USAID, but it didn't go any further. Then I quit the institute altogether. We mainly used various strains of Beaveria bassiana and Metharizium anisopliae on locusts, Colorado potato beetle, cruciferous beetles, harmful turtle, etc. Of the simplest, we worked with Nosema locusta and one local Nosema species, which was never fully identified, disappeared after I left. He took home a packet of Metharizium anisopliae spores, and has been lying there ever since, I don't know if he's alive or dead. Either my hands didn't reach it, or something else.

This post was edited by Bukashechnik - 28.08.2008 13: 07

and how was boveria singled out?

and how exactly is it known about lab? I collect any inf in Russian

This post was edited by fly-km-28.08.2008 13: 27

Isolation of pure cultures of microorganisms was carried out, depending on the type of infection, on various nutrient media using generally accepted (Weiser, Evlakhova, Shvetsova, Steinhaus, etc., well, a bunch of foreign all sorts-different), and in some cases, specially developed methods for this purpose. To obtain monospore isolates of entomopathogenic fungi and the same Boveria, a conidia suspension was prepared in sterile water. To do this, a small amount of mycelium with conidia from a 7-day culture was transferred to 5 ml of sterile water and thoroughly shaken. After a series of dilutions, the suspension was applied with a micropipette to the surface of potato-glucose agar in Petri dishes and rubbed with a spatula. The seeded cups were placed in a thermostat at 26-280 ° C. After 3 days, the plates were scanned and colonies growing from one conidia were noted. They were screened out in test tubes on Saburo dextrose agar and stored in a refrigerator at 4-60C before being used in further work. Entomopathogenic fungi were cultured by surface cultivation on agarized media (beer must-agar, Saburo dextrose agar, potato-glucose agar, etc.), surface-deep cultivation - under stationary conditions on 50% beer must, and deep cultivation-on rocking chairs using methods that were optimal for fungal development (23-26°C, pH 6.5Agarized substrates were poured 20 ml each into standard Petri dishes or 1 ml each into special microchambers (slides with a recess for the medium covered with cover glasses), liquid media-into 1.0 l or 2.0 l flasks (the ratio of the volume of the medium to the volume of the flask is 1: 5).
All this is described, as you know, for a long time, and the forum may simply not have enoughspace. I can send you a list of references that we used for our work.
It is a pity that you collect only in Russian, there is an English-language website "Journal of Invertebrate Pathology" http://www.sciencedirect.com/science/journal/00222011 , there are many different annotations to articles. Access to the articles themselves is paid, but you can also learn a lot from the annotations.
Labulbeniyevye - purely theoretical knowledge, in practice, as I have already said, I have never worked with them. The same Evlakhova, Shvetsova, individual articles of different magazines. I'll dig around at home tonight, see if I can find anything.

This post was edited by Bukashechnik - 28.08.2008 13: 51

thank you..! In principle, I know the technique in general terms.. and why exactly on the PDA?

but you didn't write down how you managed to isolate the culture initially..at least in the complex

Initially, the culture was isolated from insects in a wet chamber, but this technique is also well-known-sterility, filter paper, dist. water for humidity, etc. Then determination of sprouted mushrooms, selection of the right ones, and so on. PDA was most accessible to us simply. Old stocks from the time of sovka

Well, yes, it's familiar..so predatory animals were singled out

This post was edited by fly-km-28.08.2008 14: 24

Hoorah!!!
34 testicles!!! just collected it!!! I'm going to prepare the incubator

Congratulations on raising your offspring. Just do not feed one dog rose!

This post was edited by Bukashechnik - 08/29/2008 07: 23

Can I help you with raspberries?

fly-km, don't=)) I found out where you can get it nearby)) when there will be offspring I will inform

nearby - is it on the balcony?

no, it grows near ponds))

it's dirty!!!!

and what to feed the larvae?

I advise you to put a couple of raspberry brooms in the freezer so that you don't have to worry about food in winter. If it suddenly runs out - as an extreme option, you can buy an oak broom and soak it.

eggs are already more than 100 =))....when they stop rushing=))))

when it's about 500..

Hatches offspring, who are interested, please in PM + if possible exchange for other species, also contact)

I won't create a new theme. At me sticks began legs literally to throw off. They turn out to be quite healthy in appearance, without spots, well-fed but legless (you have to alcohol them so as not to suffer). Can this be due to dryness in the terrarium, or are there any other reasons?

Completely legless or a pair of legs remain? Don't you pull them by the legs ? and then they are nervous in this regard. Are they discarded during molting or just like that? what kind of stick insects?

Stick insects of Annam. I didn't pull my legs, that's just it. Now the situation has improved, I began to put branches with blackberries in a vessel with water (all the sticks immediately came to life visually, I used to put them in a vessel with water before, just recently somehow it was not up to it). And stick insects, if we return to the problem, being quite well-fed and lively, remained without legs at all.

Finally the forum has earned