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Drosophila content

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nikpasternak, 11.02.2018 22:42

The situation is as follows: I had a colony of fruit flies, but all of them at some point stopped hatching. The substrate is all dried up and does not absorb moisture, no matter how much I spray it, everything is useless. So I lost the colony, but I see that drosophila pupae are still hanging in some places, can I try to restore it? I just really need it, really badly. Otherwise, newborn praying mantises will die without food. If there are experts in this field, please help, give advice.

Comments

12.02.2018 0:28, Vorona

Well, I'm maintaining a collection of fruit fly lines. I won't call myself an expert, but I know something.
First, how much of your environment is for flies? It really dries up over time, the flies have nothing to eat, and they will die. Porridge should be periodically cooked a new one. With the frequency of departure of flies. At 24 degrees, I do this once every 2 weeks. In principle, it is possible for a couple of days earlier or a couple of days later, but the approximate terms are as follows. A new environment is brewed, and the flies that have flown out are thrown back on it. In 2 weeks — on a new Wednesday. They live in test tubes with the old one for a while longer.
Secondly, at what temperature do you have all this? The optimal temperature is about 25 degrees. I don't remember exactly, but at a temperature of about 30, the flies become sterile.
Well, the composition of the environment is also interesting.

12.02.2018 18:40, ИНО

And you are sure that the pupae. not empty shells from them. In general, there are a great many ways to keep drosophila and substrates for this business (and, accordingly, the duration of the cycle of changing them). If the drosophila is flying, and previously there were flights to the room, you can try to catch them by luring them with fresh water. And where do you live? In a large city, buying some small insect food should not be a problem (not necessarily drosophila, you can also "dust" a cricket, larvae of small non-burrowing cockroaches).

12.02.2018 20:53, Atropos

By the way is there one in Russia/Do you know places where you can send fruit fly lines for conservation/maintenance?

(For laboratories that don't deal with flies on a regular basis, but sometimes you need unique lines)

This post was edited by Atropos - 12.02.2018 20: 54

12.02.2018 22:01, Vorona

Atropos, the Department of Genetics at the university, for example? We periodically take them to St. Petersburg State University (in the summer, when they are on vacation, someone accidentally looks after them, they rarely don't ruin a line or two, and sometimes that's all).
Likes: 1

09.07.2018 19:26, KM2200

But I wonder who can be fed drosophila? And then I spread them, and now I don't know where to put them ))

09.07.2018 20:12, Vorona

Bogomolov. Young ones. Wingless lines — frogs, fish. This is from the one we fed. Surely you can also find someone else.
Likes: 1

09.07.2018 20:50, KM2200

Why are frogs wingless? In nature, they also catch normal ones.

09.07.2018 21:15, Vorona

So the normal ones from the aquarium will fly away. And the wingless ones (or rather, with all sorts of gnarled wings) will twitch on the surface of the water and will not fly away. The very thing that frogs need. If the flies fly away, it's not fatal, of course, but it's also not very pleasant: the windows sit up, they fly to all sorts of organic matter. Mantises can be locked up with flies in a jar, they will quickly get rid of them. Probably, you can also cover frogs from above during feeding. But we haven't tried it.

09.07.2018 21:45, KM2200

So the normal ones from the aquarium will fly away.
I didn't think of that )

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