Preparation of genitals

In crustaceans of this genus, all parameres are usually assymeric and very often have various outgrowths on the sides. I do the following. I tear off the abdomen completely, and I calmly take out all this economy from the inside. The abdomen is then calmly glued back in. Since their pygidium is relatively small, it is highly likely that both it and parts of the parameres will break. It is best to prepare the paramers themselves for the purpose of studying the inner bags on fresh material, having previously clarified them in an alkali solution, or blown them out. But this will still not give the desired effect, since until you calculate the force with which all this should be blown out, there will not be a single whole instance left. Actually, the external structures there are quite functional.
By the way, the method with breaking off the abdomen is perfectly suitable for almost all lamellate sawyers. Well, good luck in this difficult task, Mr. Sergeich.
Sincerely,..

This post was edited by Nimrod - 03/14/2007 02: 20

I wonder how and what to inflate the genitals, I have been asking this question for a long time

I have the following set for this purpose:
a pipette extended to a diameter of about 1 mm (for the smallest genitalia), an insulin syringe with a converted needle (the tip is filed and the metal layer is carefully thinned - that is, it becomes thinner), a 10 cc syringe, paraffin, cotton thread, electric stove, blade.
To begin with, I slightly cut off the basal part of the penis, put the needle through, and wrap the base tightly with thread. I insert the needle into the insulin syringe and start blowing.
If the pressure is insufficient, 10 cc is used.
I fix the blown bag over the tile until it hardens.
Paraffin - in the case of small genitals (instead of thread, the base of the penis is filled in so that air does not enter).
Briefly everything.

Sorry, I didn't log in right away - my previous message is

I have the following set for this purpose:
a pipette extended to a diameter of about 1 mm (for the smallest genitalia), an insulin syringe with a converted needle (the tip is filed and the metal layer is carefully thinned - that is, it becomes thinner), a 10 cc syringe, paraffin, cotton thread, electric stove, blade.
To begin with, I slightly cut off the basal part of the penis, put the needle through, and wrap the base tightly with thread. I insert the needle into the insulin syringe and start blowing.
If the pressure is insufficient, 10 cc is used.
I fix the blown bag over the tile until it hardens.
Paraffin - in the case of small genitals (instead of thread, the base of the penis is filled in so that air does not enter).
Briefly everything.

I glue PVA instead of thread and paraffin. Sometimes I use an aquarium microcompressor. I refused to use electrical appliances - it's easy to dry out.

Well, it's necessary to wait for the PVA to dry out. And electronics still do not feel like hands-what if it bursts? And how can you over-dry endophallus? Unless before melting or charring?

Well, it's necessary to wait for the PVA to dry out. And electronics still do not feel like hands-what if it bursts? And how can you over-dry endophallus? Unless before melting or charring?

PVA dries very quickly, and the dried bag is simply dipped in water.
It can also burst when manually inflating (it happened more than once. I don't always use the compressor, but I have to. the problem of" over-drying " sometimes pops up in the case of very old beetle specimens that were killed by unknown means. When exposed to extreme heat, the bag may shrink slightly. And if there is damage, it is very skukozhivaetsya.

Oh, and one more thing. Holotrichia has very strongly sclerotized paramers, and it is most likely impossible to blow out bags of stale material without pretreating them in alkali. Looking for H. oblita or someone else?
Sincerely,...

This is generally a separate topic "Complexity /ease/ preparation of the preparation of blown endophallus scarabeid". In comparison, even among the Madotrogus-Xanthotrogus-Chioneosoma group, the latter are blown out much more easily. And in madotrogov, 50 percent (even if the material is a year old and normally hunted) is not blown out. Apparently, the matter is in the very structure of the bag - they have a bunch of spikes and the bag itself is voluminous. Some lasiopsis are very difficult to inflate. But with hineozomami-a song. All hands do not reach the group will take. The bags produce miracles - there are a lot of local species, and trends in the structure of endophallus can be traced. I think anyone who has encountered the definition of chioneosis by external morphology, such as: pubescence of the psp, the degree of plaque development and other variable (not only phenotypically, but also age-related) signs-will understand me.

  Looking for H. oblita or someone else?

Its own

If there is a publication about this - I will be the first to print, do you mind? And more. If I am not confused, then there is some confusion in this genus, not so much with the definition as with the descriptions. I have one instance of [prope H. oblita Fald.] from Primorye, and according to all external signs, this is exactly it, and V. V. Yanushev, being with me, found it difficult to identify it more precisely when he examined it. The difficulty is that there is only one of them, and it is quite possible that this instance belongs to a different species. And he, like, was going to do an audit on this kind. Here, perhaps, you should operate with large, no, huge (if this is possible for you) series of these crunches to accurately establish the species composition of the region, with the obligatory drawings of both genitals and other body parts. If you need to view my material for review , please contact me in the personal account, we will discuss everything there.....

Do you inflate the bags yourself?

Do you inflate the bags yourself?

Yes, I myself, according to the method of O. E. Berlov, however, I already realized that this art is still far from ideal.

And the ideal doesn't exist at all. I haven't even tried it yet.

And the ideal doesn't exist at all. I haven't even tried it yet.

Interesting activity, try it. The whole trick is to inflate it as much as possible, and that, at the same time, it does not burst.

Then here's the technical question - do you use a double pear or a mouth?

Then here's the technical question - do you use a double pear or a mouth?

Neither one nor the other - with a syringe. Here is a link to the methodology.
My Webpage

No matter how much I read, I still have little idea of the process.. I would like to see it firsthand..

Neither one nor the other - with a syringe. Here is a link to the methodology.
My Webpage

and judging by the picture, you fill it with toothpaste...

Possible options? What is the best way to fill it?

Next year I will definitely try it on fresh sauces.

Yes, as I see it all goes to the fact that doing PCR analysis for hundreds of ground beetles will be easier and faster (I did it for mosquito larvae) than inflating bags. Of course, if the differences are already established.

Next year I will definitely try it on fresh sauces.

Then-good luck! And one more point - it is best to soak with ethyl acetate. With other options, it will be more difficult to make an endofallus preparation.

Another point - you do not need to cook in lye before turning out? It will dissolve all the soft tissues...

Another point - you do not need to cook in lye before turning out? It will dissolve all the soft tissues...

Edeagus, after extraction, I usually throw in boiling water for 15-20 minutes. Then I clean the base of the edeagus from soft tissues and paramer. After that, I blow out the endophallus. I didn't use boiling water in lye. This may be necessary if the beetle is contaminated with formalin or chloroform.

And why put it in boiling water? What am I asking about lye - I'm my C. sibiricus ssp. I've already boiled it down, didn't it hurt? I only had one male...

UPD: kityatok in the sense of cooking it in boiling water or just hold it?

This post was edited by Bad Den - 10/23/2007 14: 32

It seems to me that the endophallos should not be turned out already

And why put it in boiling water? What am I asking about lye - I'm my C. sibiricus ssp. I've already boiled it down, didn't it hurt? I only had one male...
UPD: kityatok in the sense of cooking it in boiling water or just hold it?

In boiling water - to soften the walls of the endophallus and to remove the associated edeagus tissues. I don't cook it. I just pour boiling water into a jar and throw in the edeagus. You should not be upset, Bad Den, I think that in your case everything should be fine. Just how much % alkali solution did you use and how long did you cook it?

And why put it in boiling water? What am I asking about lye - I'm my C. sibiricus ssp. I've already boiled it down, didn't it hurt? I only had one male...

UPD: kityatok in the sense of cooking it in boiling water or just hold it?

I always process in lye. even fresh beetles. and with the old ones, without such processing, you should not contact them.

Yes, as a filler - toothpaste.

I don't fill it with anything. Except for the air - and nothing, they look great...

Sorry, KDG, maybe ground beetle sacks are still different from barbel sacks? And with them in any way without filler? Or did the ground beetles cheat too?

This post was edited by omar - 10/23/2007 15: 23

Just how much % alkali solution did you use and how long did you cook it?

I did not cook, but rather kept it in a 9% r-re KOH heated in a water bath for 10 minutes.
I messed up my concentration...

I haven't reached the endophallus of barbels yet. But as far as I can tell, they are simpler in shape. The filler is good because it hardens, it maintains the shape of the endophallus. In addition, with the filler, it becomes opaque and it is more convenient to observe its structure and armament. Whether the form of the endophallus sags over time (when inflated with air and dried) or not - I don't know, I don't have such drugs. In general, this is a matter of taste and habit.

I did not cook, but rather kept it in a 9% r-re KOH heated in a water bath for 10 minutes.
I messed up my concentration...

According to Shilenkov V. G.-boiling in 15% alkali for 2-3 minutes. I can't say anything else yet.

In general, we are completely confused in this topic - we need to ask the moderator to take it all down in a new topic - "Making endophallus preparations".

Sorry, KDG, maybe ground beetle sacks are still different from barbel sacks? And with them in any way without filler? Or did the ground beetles cheat too?

they differ mainly in the fact that preparations are much more difficult to prepare.
With a filler such as toothpaste, deformations are given. In addition to barbels, ground beetles, leaf beetles and plates were also blown. I tried zernovok, but so far without success, the small ones are too reptiles...

But as far as I can tell, they are simpler in shape.

Well, I'm not sure here.. I can throw an article on "soap", there are a lot of pictures and everything written about sawyere bags.

Well, I'm not sure here.. I can throw an article on "soap", there are a lot of pictures and everything written about sawyere bags.

Thank you, KDG, it would be interesting to read. safalex@rambler.ru
The fact that endophallus in barbels is more difficult to dissect, of course, I believe, as a specialist! I don't have any experience with them, but I'll try them out in the near future.
And at the same time - tell me what you use as an alkali for cooking?