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Preparation of genitals

Community and ForumEntomological collectionsPreparation of genitals

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10.04.2010 4:33, Konung

I think that Barko could organize a master class - because his drugs are great

by the way, I would not refuse smile.gif

10.04.2010 20:32, barko

Roma, thank you for such flattering reviews of my work.
I am ready to share my experience with everyone. But I don't have enough time for a full-fledged master class. Therefore, at first, I will simply answer all the questions that anyone has about the technique of preparing different types of drugs and making euparal preparations. Over time, I will try to make a convenient tutorial.

(Damn, Roma, I don't understand all your allegoriessmile.gif)

10.04.2010 20:41, barko

I haven't seen all of his works, but judging by what is displayed in this topic (post # 99, # 129, etc.), this is probably the case...
By the way, what about large, or" branched " genitals, such as in the male Hipparchia pellucida (Stauder, 1923)? I can't imagine how they can be "rolled up" in euparal? Although, to be honest , I myself have NEVER tried to work with euparal, and I have no right to judge, since I have only seen it in a bottle... I'm still a "caveman"...
You can handle large genitals. It is more difficult with "branched" ones. So far, there is no good solution for the genitals of male bull whiskers. They are voluminous. Their structure is such that they cannot be placed in the same plane under the cover glass without deformation. I think a suitable solution will eventually come.

10.04.2010 20:57, KDG

You can handle large genitals. It is more difficult with "branched" ones. So far, there is no good solution for the genitals of male bull whiskers. They are voluminous. Their structure is such that they cannot be placed in the same plane under the cover glass without deformation. I think a suitable solution will eventually come.

Oleg, and if you roll up the glass with a hole? (sorry that I don't fit into my diocese:)).

10.04.2010 21:17, barko

Oleg, and if you roll up the glass with a hole? (sorry that I don't fit into my diocese:)).
Yes, Denis had such an idea. I even found slides with a hole in them. There are a number of obstacles here. For large daytime images, the depth of the hole is not enough, the hole gives optical distortions when photographing, and the cost of one such glass is about 1.2 euros. This is not the way to go. Thank you for showing interest and taking part!

10.04.2010 22:16, KDG

Yes, Denis had such an idea. I even found slides with a hole in them. There are a number of obstacles here. For large daytime images, the depth of the hole is not enough, the hole gives optical distortions when photographing, and the cost of one such glass is about 1.2 euros. This is not the way to go. Thank you for showing interest and taking part!

The fact is that the holes are different, both in shape and depth, and in the type of surface. There are matte ones, they probably distort, and there are polished ones. I just use the holes myself to view endophallus and am quite satisfied. And as for the price, for 1,2 oiropcs, they not only should not give distortion, but they are also required to straighten the drug and dance chardash! Buy Russian for 6 rubles!

10.04.2010 22:32, barko

The fact is that the holes are different, both in shape and depth, and in the type of surface. There are matte ones, they probably distort, and there are polished ones. I just use the holes myself to view endophallus and am quite satisfied. And as for the price, for 1,2 oiropcs, they not only should not give distortion, but they are also required to straighten the drug and dance chardash! Buy Russian for 6 rubles!
For 6 rubles.? eek.gif Give me two. I mean, buy me a pack or two if you don't mind.

10.05.2010 2:03, barko

.. In fact, the same parasitofauna of insects is almost not studied. ...
In continuation of the topic ... It's not the first time I've found a living organism in the belly of a shovel. It looks a bit like a crustacean.

001.jpg

10.05.2010 5:36, Konung

In continuation of the topic ... It's not the first time I've found a living organism in the belly of a shovel. It looks a bit like a crustacean.


eek.gif

10.05.2010 6:52, RippeR

parasites love everyone..

10.05.2010 15:46, Zlopastnyi Brandashmyg

In continuation of the topic ... It's not the first time I've found a living organism in the belly of a shovel. It looks a bit like a crustacean.


A larva? I can show the photo to our hymenopterologists.

I didn't see it right away: it doesn't attach to the trachea at the back end? If I remember correctly, this is how many Parasiticas are attached.

This post was edited by Zlopastnyi Brandashmyg - 05/10/2010 15: 48

28.06.2010 21:57, Mantispid

And what to do if there is no alkali and there is no way to get it? Is there anything you can replace it with? For example, I heard that the so-called "Mole" for cleaning pipes is 70% alkali. Can it be used?

29.06.2010 0:02, okoem

And what to do if there is no alkali and there is no way to get it?

If it is not possible to get lye, then you can just buy it. The first thing Google gave out: Chemical raw materials from a warehouse inSaratov: caustic soda (),
http://www.megasklad.ru/lots/view/545877

29.06.2010 6:06, Mantispid

They are sold only to law firms, but they are not sold to individuals - the law umnik.gif

This post was edited by Mantispid - 29.06.2010 06: 07

29.06.2010 11:23, Necrocephalus

Lye is also sold to individuals. Including chemical stores (which are available in every major city), by weight.
"Mole" is a mixture of sulfates and alkalis, I think it is suitable for processing genitals - for testing, you can practice on something not particularly valuable.

29.06.2010 13:47, barko

Lye is available in any city. I need to ask around in chemical stores. In extreme cases, you can order via the Internet from sellers of entomological equipment http://www.insectnet.eu/microscopy_chemicals.php

30.06.2010 6:49, rhopalocera.com

You can buy KOH in any entomological online store. With its meager consumption, a standard package of 200 grams is enough for a year or two. So 15 euros is not the price for it. I recommend Entosphinx

04.07.2010 23:11, Alexandr Zhakov

And what to do if there is no alkali and there is no way to get it? Is there anything you can replace it with? For example, I heard that the so-called "Mole" for cleaning pipes is 70% alkali. Can it be used?

I had to determine the dustpan at home, there was no lye, I experimented with "Mr. Cleaner" - a means for removing fat contamination.
Composition:water, surfactants, alkaline and organic solvents, emulsifiers. The result is acceptable, the scoop boiled for 3-5 minutes, as in alkali. I didn't notice any difference.
Likes: 2

11.07.2010 20:12, barko

I had to determine the dustpan at home, there was no lye, I experimented with "Mr. Cleaner" - a means for removing fat contamination.
Composition:water, surfactants, alkaline and organic solvents, emulsifiers. The result is acceptable, the scoop boiled for 3-5 minutes, as in alkali. I didn't notice any difference.
All this is true, but you need to be careful with solvents and other active substances, they can affect the safety of the genitals during subsequent storage.

11.07.2010 22:14, Nosferatumyia

And what to do if there is no alkali and there is no way to get it? Is there anything you can replace it with? For example, I heard that the so-called "Mole" for cleaning pipes is 70% alkali. Can it be used?

Mole can be used in principle, but it also discolors, and fills the drug with bubbles, which then do not want to dissolve for a week...

12.07.2010 11:41, Mantispid

I experienced "Mr. Muscle" yesterday. The result is quite good, although I have nothing to compare it with. Of the minuses, I note a strange film formed after cooling, but it is easily removed.

This post was edited by Mantispid - 12.07.2010 11: 42
Likes: 1

15.11.2010 20:05, косинус

Hello
, do not tell me.
Well, with the storage and preparation of the genitals of ground beetles and barbels, it is clear. And how to dissect the genitals of smaller beetles, such as nutcrackers. And how to store them (stick them on dies or how not to be otherwise)
Sincerely Konstantin.

17.11.2010 0:39, RippeR

You can stick it on a die or triangle, but I think you can also put it in glycerin like butterflies.

07.02.2011 14:05, Valentinus

Friends!
I offer you my own method of cooking the genitals of butterflies.
The method is very simple, cheap and affordable. jump.gif
You need: an aroma lamp (bought at any pharmacy) with a flat top, candles-tablets, liquid - MOLE (in any household), cover glass.
Pour the mole into the aroma lamp, put the belly, cover it with glass and light a candle. EVERYTHING!!!
Do not use any test tubes and chemical alkali!!!
It is very easy to see the degree of readiness.
picture: __________.jpg

07.02.2011 15:14, BRM

Can you see the result of such frying?

07.02.2011 16:35, Valentinus

Can you see the result of such frying?

Unfortunately, I can't take a picture, there is no installation. But you try it, I think you'll like it. smile.gif

07.02.2011 20:33, Zlopastnyi Brandashmyg

I have long abandoned the actual cooking - it is dangerous for the drug (test tubes crack, very easy to digest, etc.) and inconvenient (messing around with an open fire in the room, especially considering that I usually work at home...). The drug is immersed in an alkali solution and kept for the right time (usually 12-24 hours, sometimes more). IMHO, the only negative is a long wait.
Likes: 1

07.02.2011 21:00, okoem

Need: aroma lamp (available at any pharmacy)

And how long does it take for the alkali to boil?
Looking at the installation, it seems that the boiled lye will get out... In vitro climbs, why not here?

test tubes crack,

I had two test tubes - one cracked after a dozen or two brews, and the other is still alive. The difference is the second one is more thin-walled.

This post was edited by okoem - 07.02.2011 21: 13

07.02.2011 21:12, Kharkovbut

I cook in a water bath (intermediate versionsmile.gif). Cooking lasts 30-50 minutes.

07.02.2011 22:57, palvasru4ko

I have long abandoned the actual cooking - it is dangerous for the drug (test tubes crack, very easy to digest, etc.) and inconvenient (messing around with an open fire in the room, especially considering that I usually work at home...). The drug is immersed in an alkali solution and kept for the right time (usually 12-24 hours, sometimes more). IMHO, the only negative is a long wait.

I've been using this method myself for a long time. As a rule, I can only work in the evenings. Today I "soaked" it, tomorrow I isolated it and cleaned it. In 10 - 15% NaOH, the genitals of the male of average thick-headed cats "turn sour" for a day to the state of normality, only after two or three days they become too soft and light. In general, it is difficult to" bury " the moment, you need to try here. For those who have nowhere to hurry , this is the most reliable method.
Likes: 2

07.02.2011 23:33, Zlopastnyi Brandashmyg

I cook in a water bath (intermediate versionsmile.gif). Cooking lasts 30-50 minutes.


I also used a water bath during the "transition period". Plus, compared to "standing the drug", only one thing - the process is faster. But there is much more fuss.

08.02.2011 0:37, okoem

In general, it is difficult to" bury " the moment, you need to try here.

Well, it's like a coma. If I put this case up for a day, I'll remember it in a week (verified!). So for me, the method is not suitable for ambiguous purposes. I prefer to boil for a minute - and you're done. smile.gif

08.02.2011 11:09, Valentinus

And how long does it take for the alkali to boil?
Looking at the installation, it seems that the boiled lye will get out... In vitro climbs, why not here?

This method is completely safe. The liquid practically does not boil and does not climb anywhere. Nothing cracks and there is no need to constantly monitor the situation. I used to cook both in test tubes and by the cold method, but this one is a bomb! smile.gif
Just need to pick up an aroma lamp about the same as in the picture. Some are made with twists and candles are lit in them.
Try it, then drink to my health. beer.gif

08.06.2011 13:50, barko

Can you tell me the best way to cut cover glasses? You need narrow strips with parallel edges. Sometimes you just need to cut an even piece.

08.06.2011 14:53, Proctos

I used diamond-coated canvases, they are used for example in jewelry tools, for small jigsaws on metal. You can take a used canvas, break it into several pieces and carefully scratch it with a ruler. The result is small squares made of large forged glass. For filling individual small parts.
Likes: 2

21.09.2011 7:55, rhopalocera.com

I have long abandoned the actual cooking - it is dangerous for the drug (test tubes crack, very easy to digest, etc.) and inconvenient (messing around with an open fire in the room, especially considering that I usually work at home...). The drug is immersed in an alkali solution and kept for the right time (usually 12-24 hours, sometimes more). IMHO, the only negative is a long wait.



Pyrex test tubes. None cracked yet smile.gif

21.09.2011 14:30, lepidopterolog

Test Tubes Are Soviet. Also no cracks smile.gif

26.09.2011 22:07, John-ST

Pussy did not cook, but in my experience test tubes burst if they are heated unevenly 50/50, and if the liquid in an unevenly heated test tube boils, then almost 100% result, thick-walled tubes are difficult to quickly and evenly warm up.
I used only Soviet and Polish Soviet period test tubes, so far not a single one has burst at home, even with calibration.

27.09.2011 10:51, scar

Can you tell me the best way to cut cover glasses? You need narrow strips with parallel edges. Sometimes you just need to cut an even piece.

Ideal for accessibility option (in my opinion) - a circle for opening apmul.
Likes: 1

27.09.2011 11:02, barko

I found a small piece of canvas with a diamond coating and successfully apply it.

010.JPG
Likes: 1

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