Preparation of genitals

Thank you, KDG, it would be interesting to read. safalex@rambler.ru
The fact that endophallus in barbels is more difficult to dissect, of course, I believe, as a specialist! I don't have any experience with them, but I'll try them out in the near future.
And at the same time - tell me what you use as an alkali for cooking?

Sent it.
Alkali-CON. concentration varies from 5 to 30%.

KDG:
So what's the secret? Without filler, do everything the same? And after inflating - tie, seal, what? How do they keep their shape, and at what cost?

KDG:
treator@gmail.com with thanks

KDG, this article?
"The inner sac of the aedeagus of woodcutter beetles (Coleoptera: Cerambycidae): morphology, nomenclature of structures, taxonomic significance."

This post was edited by Bad Den - 10/24/2007 19: 18

KDG, this article?
"The inner sac of the aedeagus of woodcutter beetles (Coleoptera: Cerambycidae): morphology, nomenclature of structures, taxonomic significance."

ага

KDG:
treator@gmail.com with gratitude

Sorry, I don't understand. Should I send it to you too?

KDG:
So what's the secret? Without filler, do everything the same? And after inflating - tie, seal, what? How do they keep their shape, and at what cost?

They keep their shape because they are inflated and dried up.

So at the end, after inflating apply? Tie them up, seal them at the end, or they don't lose their shape just like that?

So at the end, after inflating apply? Tie them up, seal them at the end, or they don't lose their shape just like that?

don't lose

Article about blowing out endosomes in bedbugs

File/s:



aedeagi.pdf

size: 199.15 k
number of downloads: 17
12.11.2007-26.11.2007

I have blown at ground beetles (Harpalus, Zabrus, Carabus). First with water, and then "drained" the water and inflated it with air and dried it. But what about the smaller ones???????? Such as Microlestes, Syntomus??? At least turn it out...

This post was edited by Adamov - 13.11.2007 13: 07

I have blown at ground beetles (Harpalus, Zabrus, Carabus). First with water, and then "drained" the water and inflated it with air and dried it. But what about the smaller ones???????? Such as Microlestes, Syntomus??? At least turn it out...

The article attached above discusses the use of glass capillaries for blowing out small edeaguses. The fact is that you can pull the capillary over the flame of an alcohol lamp to any diameter, then, breaking off at the end, you get a microneedle. Insert such a capillary into the nozzle from a regular needle of an insulin syringe, secure it with a rubber sleeve for tightness, put it on the syringe and blow it out.

The article attached above discusses the use of glass capillaries for blowing out small edeaguses. The fact is that you can pull the capillary over the flame of an alcohol lamp to any diameter, then, breaking off at the end, you get a microneedle. Insert such a capillary into the nozzle from a regular needle of an insulin syringe, secure it with a rubber sleeve for tightness, put it on the syringe and blow it out.

Thanks!
And how to fix such a small edeagus on the tip of the capillary?

Thanks!
And how to fix such a small edeagus on the tip of the capillary?

You can fix the edeagus at the end of the capillary with a transparent nail polish, it dries quickly, provides tightness and strength of fixation (a small edeagus cannot be held with your fingers when inflating). After inflating with water, air and after drying the inflated endosome, the varnish is removed from the walls of the teka (penis? median lobe?) it is removed with a preparation needle: it easily peels off if the preparation has not been dried for more than 10-12 hours. By the way, for blowing air, I recommend a plastic syringe of Janet: the plunger rests on your chest and with both hands you pull the syringe towards you with all your strength, while holding the end of the capillary with the glued edeagus in a jet of hot air (for example, from a hair dryer). Because of the very small inner diameter of the capillary, it is necessary to develop a very large air pressure, otherwise the endosome will not be straightened, and the varnish is irreplaceable here.

It all looks something like this (I once took photos for one report). At the end of the syringe, Janet can see an adapter made from an insulin syringe, which can be hermetically attached by means of a piece of rubber tube of the desired diameter.

Pictures:

method1.jpg — (99.7к)


method2.jpg — (14.59к)

say I'm a newbie just....why even dissect your genitals??

say I'm a newbie just....why even dissect your genitals??

Many insect species are correctly identified only by their genitals. For example, this technique is used in determining the species of moths (mottled lepidoptera). In general, this is a common technique. Although in some groups, this method is minimized if the determination is carried out well for other morphological features. Differences in the genitals are an important feature for distinguishing a new species. It is considered that the genitals of each insect species are unique (the definition mainly uses the genitals of the male, since they have many specific outgrowths and special shapes). For example, there is a species A that has a certain morphological shape (color, hairline, outgrowths, etc.), but this species is not evenly distributed - for example, in Europe and North America. America (disjunctive - broken, area). Externally, they are very similar, with minor differences in color. However, when we looked at their genitals (and they are digested in alkali before looking at them), it turned out that they (genitals) are very different in these species. Consequently, two species were distinguished from species A - A occurring in Europe, and B occurring in North America.

Differences in the genitals are an important feature for distinguishing a new species. It is considered that the genitals of each insect species are unique (the definition mainly uses the genitals of the male, since they have many specific outgrowths and special shapes).

Who counts as? This is a misconception. Genitalia in many groups are very informative structures due to the complexity of their structure, but cases where they do not differ (and not only the genital segments, paramers, external genitalia, but also internal ectodermal genitalia) in similar species are not uncommon. You should not make a fetish out of any group of traits, be it genes, chromosomes, or genitals. In taxonomy, you should use any working attributes. The ancient view that the genitals of male and female insects fit together like a key to a lock and provide mechanical isolation of species has been repeatedly challenged by the example of very different groups. Apparently, this principle works, but not always.

Who is interested - here is a small study on the problem of "key-lock" on the example of one kind of bedbugs-soldiers, published recently in the"Review".

And in terms of flushing - it's not so difficult to add a fairy thread to the water)))
Yes, here I still remembered such thing about alcohol - after all with genitals in alcohol svosem badly tsanovitsya. Everything shrinks down to the barbie-size, and probably only David Blaine will be able to shred it back. That is, I mean, if you need to blow the bag (for ground beetles there, or for weevils).
Or am I wrong again?

I don't know how much I pulled it, but I think everything was fine with them. Endophallus, though not blown, but I don't think that everything is so bad there. Besides, I remember them being blown out with alcohol sometimes. Or not? After KOH is blown out, does alcohol really affect them more harshly?

This post was edited by Fornax13-23.08.2008 20: 10

I don't know how much I pulled it, but I think everything was fine with them. Endophallus, though not blown, but I don't think that everything is so bad there.

It is no more difficult to pull the aedeagus of a beetle starved with alcohol than that of a beetle killed by something else. However, it is an order of magnitude more difficult to make a preparation of an inverted endophallus. If there is a desire, compare it.

It is no more difficult to pull the aedeagus of a beetle starved with alcohol than that of a beetle killed by something else. However, it is an order of magnitude more difficult to make a preparation of an inverted endophallus. If there is a desire, compare it.

It will be possible. Some time at your leisure. But I don't really need to blow the bags yet - you can see the weapons in the small change. Is there a difference between" soaked in alcohol "and" soaked in alcohol " in terms of manufacturing endofallus preparations?

There is no difficulty in twitching the aedeagus after alcohol, but the difference will be in blowing.
The bag becomes too hard after alcohol. Somehow it needs to be soaked. In a solution of caustic potassium, the bag does not harden, but soft tissue destruction occurs.
I haven't heard anything about blowing with alcohol. They usually blow with water, air, or toothpaste. I know it's silicone, too, but I've never tried it myself.

Is there a difference between" soaked in alcohol "and" soaked in alcohol " in terms of manufacturing endofallus preparations?

Well, I can't say anything about soaking in alcohol - I've never tried it, and it probably doesn't make sense. Zamarivayu only ethyl acetate. Endophallus (at least Carabidae) inflate even without boiling in alkali, pre-throw edeagus in boiling water for 10-15 minutes. However, I use toothpaste as a filler.

Yes, I also poison with ethyl acetate. I haven't tried blowing endophallus, and I don't think I'll have to in the near future - basically, there's nothing to look at there...
By the way, how to make an endofallus preparation from very small objects? In some work I saw drawings of inverted bags of small aleocharines.

 
By the way, how to make an endofallus preparation from very small objects? In some work I saw drawings of inverted bags of small aleocharines.

Unfortunately, I don't know the technology for particularly small representatives, I haven't tried it yet. It all depends on the diameter of the basal opening of the aedeagus, the thickness of the needle should not exceed it. Perhaps they use a completely different method.

That's the whole point... It seems that you can squeeze out of someone, but how...

The technology depends on the method. If a filler (paste, glycerin-gelatin mixture, etc.) is used to fix the maximum swollen endophallus (and it is only in this case species-specific), then filling occurs only through a needle. If you blow with air, followed by drying , you can blow with a "pear" and attach the basal part of the edeagus inside its inlet or adapter. Perhaps small things and blow.

This post was edited by Entalex - 08/23/2008 23: 39

People, here about dies, or where?

Here already about everything))))
And about blowing bags of small things: this requires thin glass capillaries made of low-melting glass, which can be stretched over the fire to the desired diameter. And then it blows air and dries. I know that aquarium compressors are used for this purpose.
This is the technology I know of. Maybe there are others.

Who can advise you on how to store your genitals? In the museum, under the leaf wrappers, I saw something like plastic dies with pits (depressions). They have some kind of liquid in them, and there, as I understand it, the genitals themselves. With staffs, a different picture, next to them cones with a diameter of 3 mm and a height of 2-3 cm. But I don't like it so much, and secondly, I can't get so many cones. I need the genitals to be in working order and at the same time in good condition. Who can advise what from the best available pharmacy material. I did this with butterflies. Below the copy, I took a die on it a drop of sugar syrup, and on them genitals (in which case you can immediately soak), but sugar syrup = happiness for kozheedov. So I want to know what can be used.
Advised glycerin - but I don't know where to get it.
Clove oil - but I don't know how it can be used for this purpose or not. In general, write who does how.

I keep them in blisters (that's what they call it, I think) made from small tablets (such as glycine) in glycerol (which I buy in pharmacies), pinned on a pin with a copy; at the department they store them in tapes cut into small pieces for developing photographic film "Correx" (now they are no longer produced) - there are such the recesses are small, very convenient, also in glycerin. I have heard that someone pours it into sugar syrup, but IMHO it is less convenient...

And what is glycerin sold in pharmacies? Or you buy preperaty glicirinsoderzhashchy, simply at me as that weakly associates glycerin as medicine any, rather as explosive in-vo. Therefore, the question is whether to ask in the pharmacy just glycerin, or preparations based on it. The pill blisters themselves - do you buy pills specifically for this purpose, or are they sold where? But the film Corrector saw in the museum, thank you for suggesting it, you will have to ask for it, it's still lying around.

Glycerin - in its pure form, sold in small vials as ammonia. Empty blisters are unlikely to be sold anywhere, so I just take the used ones. Wow, the Correction is just lying around, lucky! I don't know where to get it, for example.

And what is glycerin sold in pharmacies? Or you buy preperaty glicirinsoderzhashchy, simply at me as that weakly associates glycerin as medicine any, rather as explosive in-vo. Therefore, the question is whether to ask in the pharmacy just glycerin, or preparations based on it. The pill blisters themselves - do you buy pills specifically for this purpose, or are they sold where? But the film Corrector saw in the museum, thank you for suggesting it, you will have to ask for it, it's still lying around.

Glycerin is sold in any pharmacy, it is not an explosive substance. Blisters are not sold separately, of course, they are just a package of medicines. Ask "Bromhexin" (but not foreign production), it costs a penny, and there are a lot of cells.

Just do not forget to close the blisters on top during long-term storage. In the simplest version, simply seal with tape. If left open, then, firstly, it dries, and secondly, if handled carelessly, the genital can fly out

When sealing with adhesive tape, it is important that the edges to which the actual tape is glued are dry, i.e. do not pour glycerin into the cell "on top".

I have seen "bourgeois things": a small test tube with a soft cork. The drug is placed in a test tube and through the cork is pinned on a needle under the impaled insect. It looks very nice, but the price exceeds the possibilities.

I myself have recently preferred to store genital complex preparations in eppendorfs. This is due to the fact that recently I prefer to work with alcohol material, and when processing foreign collections, they often set a condition - a collection of drugs in test tubes separately.

Nitroglycerin is explosive, but this is completely different. I bought ordinary glycerin at the pharmacy (by the way, with potassium permanganate it turns out to be a cool thing - spontaneous combustion ). I've heard that someone uses it to wipe their hands-very useful.. I don't know much about other appointments.

Glycerol and sugar have their drawbacks. If the box gets rotted or falls or is turned over, etc., the glycerin may fly out with the drug. On the one hand, it's dangerous, because then it can be difficult to figure out where whose ones are. This is the advantage of sugar syrup with a very small amount of sugar, as it dries up and nothing will fall out, and then dissolves with water if necessary.. You're not going to work on your genitals too many times anyway.. And just dissected genitals can be photographed through a microscope, so as not to pull many times.

A label with a locale in London, but with a definition in Budapest. In general, you can sort it out in parts like this

This post was edited by Trofim - 21.01.2009 16: 30

This is not very good. I myself saw a type of Khnzorian from Armenia with a postscript on the label - "edeagus in Paris".

It's an endless argument...
Apparently, storing both the copy and the drugs "on the same pin" is preferable, but not always convenient. Separately stored drugs are more convenient, but they tend to get lost.