Preparation of genitals

Just ampoules at every house lying around; -)

another token from the abdomen of a Chinese butterfly

another token from the abdomen of a Chinese butterfly

The TV series "Elien and the boys" Is still like zoology, parasite?

It looks like some kind of crustacean.

In Southeast Asia, there are crustaceans that are parasitic on cockroaches.
Even vaguely similar to someone's embryo. Is it big at all?

In Southeast Asia, there are crustaceans that are parasitic on cockroaches.
Even vaguely similar to someone's embryo. Is it big at all?

Size-1.25 mm. Is there a reference to the parasite crustacean?

Definitely a parasite, but what a parasite. The lye ate it all away. All the soft tissues dissolved.



This post was edited by barko - 09.10.2011 19: 15

Strepsiptera?

http://www.artsci.uc.edu/collegedepts/biol...repsiptera.aspx

In any case, "pointing your finger at the sky", since almost nothing is visible in the photo/nothing is left.

In Southeast Asia, there are crustaceans that are parasitic on cockroaches.
Even vaguely similar to someone's embryo. Is it big at all?

Can I get a link? I've never heard of anything like it.

Today I asked S. A. Belokobylsky - he could not recall any cases of hymenopteran parasitization on adult butterflies.

I found something on this subject in Atalanta

[attachmentid()=125802]

[attachmentid()=125803]

Surely everyone uses such devices, but nevertheless...

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2 scar

Micro-samples only. From blisters and other poorly closing equipa refused completely. Cocktail pipes are better, but also not the same. And preparations on paper strips (that in glue, that in sugar) - IMHO mockery of future research (and researchers).

I totally agree. I used to use pill blisters, but now I've given up. For storing genitals and other structures, I use eppendorfs of different calibers. Since even good eppendorfs are periodically depressurized, I store eppendorfs in a larger container, also filled with alcohol.

This post was edited by Zlopastnyi Brandashmyg - 18.11.2011 20: 11

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2 scar
Micro-samples only. From blisters and other poorly closing equipa refused completely. Cocktail pipes are better, but also not the same. And preparations on paper strips (that in glue, that in sugar) - IMHO mockery of future research (and researchers).

I totally agree. I used to use pill blisters, but now I've given up. For storing genitals and other structures, I use eppendorfs of different calibers. Since even good eppendorfs are periodically depressurized, I store eppendorfs in a larger container, also filled with alcohol.

It can be stored in test tubes if it is not important to preserve the volume of structures. In the case of moth-bearing butterflies, only euparal is suitable. In a permanent euparal preparation, I permanently preserve the volume of the inverted vesica of males or the copulatory bag of females, and at any time I can take a photo or consider the features of the structure. In glycerin or alcohol, an inverted vesica or bursa capulatrix looks like a shapeless pile of snot. In addition, constantly removing the genitals from the test tube, placing them on a slide, and putting them back in the test tube inevitably leads to damage and eventually confusion.






The twisted and inflated vesika was forever frozen in euparal. The natural position of all its parts is fixed. Very convenient.

In the case of moth-bearing butterflies, only euparal is suitable.

Oleg, is there something laid between the slide and cover glass to preserve the volume?

Oleg, is there something laid between the slide and cover glass to preserve the volume?

Cubes made of plastic (plastic champagne stopper) are placed under it. Methyl alcohol stores a supply of such cubes, which can be cut to size for a specific case.

It can be stored in test tubes if it is not important to preserve the volume of structures. In the case of moth-bearing butterflies, only euparal is suitable. In a permanent euparal preparation, I permanently preserve the volume of the inverted vesica of males or the copulatory bag of females, and at any time I can take a photo or consider the features of the structure. In glycerin or alcohol, an inverted vesica or bursa capulatrix looks like a shapeless pile of snot.

In the case of my groups, the situation is just the opposite - it is almost impossible to study the structure of the genitals on a permanent drug.

In addition, constantly removing the genitals from the test tube, placing them on a slide, and putting them back in the test tube inevitably leads to damage and eventually confusion.

Yes, this is a fundamental disadvantage of storing drugs in test tubes. I don't know how to deal with it.

The twisted and inflated vesika was forever frozen in euparal. The natural position of all its parts is fixed. Very convenient.

Indeed, from my point of view, a wonderful drug.

I wonder if the modern technology of manufacturing beetle genitals has also progressed so far? Does anyone have a sample of a masterpiece in this regard?

I wonder if the modern technology of manufacturing beetle genitals has also progressed so far? Does anyone have a sample of a masterpiece in this regard?

Dicelosternus coralinus, lateral view of endophallus

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Copra - Spanish and Lunar

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A short video course on working with the genitals of butterflies. 350 megabytes, web quality, about 23 minutes. http://www.rhopalocera.com/genit.mp4

A short video course on working with the genitals of butterflies. 350 megabytes, web quality, about 23 minutes. http://www.rhopalocera.com/genit.mp4

It is very dangerous to boil a test tube with alkali on an open fire. Don't take that risk. It is better to use a water bath.

It is very dangerous to boil a test tube with alkali on an open fire. Don't take that risk. It is better to use a water bath.

What is the danger? I prepared more than one thousand micro-preparations in this way, the test tubes "shot" only the first time, due to inexperience.

What is the danger? I prepared more than one thousand micro-preparations in this way, the test tubes "shot" only the first time, due to inexperience.

The test tube may crack due to an accidental scratch e.g.

The test tube may crack due to an accidental scratch, for example.

This has never happened before. Although when boiling with a test tube and beat on an alcohol lamp . I use only thick-walled heat-resistant test tubes Pyrex

The test tube may crack due to an accidental scratch, for example.

It is for this reason that I refused to boil and switched to using a water bath. After a while, he also refused a water bath, switching to aging in alkali. The only downside is that it takes longer.

To rhopalocera: I am very happy for you, but, unfortunately, not all entomologists have test tubes with "diamond coating and mother-of-pearl buttons".

It is for this reason that I refused to boil and switched to using a water bath. After a while, he also refused a water bath, switching to aging in alkali. The only downside is that it takes longer.

To rhopalocera: I am very happy for you, but, unfortunately, not all entomologists have test tubes with "diamond coating and mother-of-pearl buttons".

They are absolutely not a problem to buy. They cost only 5 euros for a dozen. Google to help .

like a collective farm, but it has been working for 15 years without failures...

And I like some thing the guy has in this video
http://science.kqed.org/quest/video/chasin...finding-darwin/

Something like a plastic jar that boils lye on a tile that doesn't melt plastic. Or am I missing something?

This post was edited by Mantispid - 12/21/2011 15: 47

may I live like this

It works like a collective farm, but it has been working smoothly for 15 years...

I have an even more collective-farm-like main thing.

......side view

It works like a collective farm, but it has been working smoothly for 15 years...

Have you been cooking butterflies for 15 years, which is very solid, or does the design last so long?

Have you been cooking butterflies for 15 years, which is very solid, or does the design last so long?

and Varya (Elena Mikhailovna Antonova taught her, God rest her soul) and the design serves so much-I did it myself and for myself (a piece of ski pole and a 2.5 mm aluminum sheet), only the new mug (5 years old) , the old one after 10 years of active use, the handle fell off

Frequently asked question - how to turn out the scales at the scoop. Different subfamilies have their own structural features and even different groups have nuances, but for most scoops with a simple structure of scales, the principle is the same.

Cleaned copulatory apparatus of scales, connective membranes, muscle residues and other things.

Separating the aedeagus


This photo shows the remnants of muscle on the ductus ejaculatorius. Their accumulation can often prevent eversion. If possible, we remove them with tweezers or a dissecting needle and a hook at the end.



Push the ductus ejaculatorius into the edeagus with a blunt needle.



Insert the needle of the syringe into the edeagus



We take the needle of the syringe with our fingers


We move our fingers so that we grab the tip of the edeagus (tsekum) and press it against the needle of the syringe


We give the water pressure. Unclench your fingers.



We transfer the edeagus with the scales turned out in a bath with liquid

Once again, about the importance of removing the remaining muscles from the aedeagus before turning the scales.

Here's what happens if you don't.

The muscles gather in a ball and prevent the distal part of the vesica and ductus ejaculatorius from everting.




If you give a larger water pressure, then in most cases, the wall of the scale breaks through and its non-inverted part goes out through the hole.



From this point on, the inverting process is incredibly complicated. Somehow it is possible to push the tip of the scale inside and partially remove the lump of muscle.



A few more successful manipulations and the weight is completely turned out. You can prepare for the New Year's feast!

Do not attempt to turn the scale out by simply pressing the plunger of the syringe. Very often, the vesica inside the aedeagus is formed unsuccessfully for eversion. The ductus ejaculatorius can twist and then it will never come out. If you give more water pressure, the weight will break in the thinnest place. Most often, a hole is formed near Karina. The walls of the vesica at this point are a thin membrane.

The photo shows that with excessive water pressure in the hole formed, ductus ejaculatorius came out with water on the rifle. Now you need to push it back inside the scales with a thin needle and blow it out again.

you went what? why do you pickle beetles ' pussies?

you went what? why do you pickle beetles ' pussies?

to eat vodka with them on long winter evenings. what do you think, you dirty pervert?