Preparation of genitals

you went what? why do you pickle beetles ' pussies?

We still know how to inflate them

p.s.
stas, well done, cool annealing

Very interesting was the shape of the bag in manticores, can anyone blow them - I really wonder if there is a difference in other species, since the edeagus look very similar, but here is the second part with the whip-very bad - - high requirements for the material-than etched and when. True, I took a quick photo with my mobile phone.
this is Manticora livingstoni

Pictures:

edeagus_Manticola_livingstoni_SIA_01.jpg — (172.29к)

The drug looks very nice!

But apparently it is very fragile? Are you not afraid to stick them on, or is there no other way to store them?

No, not very fragile, but caution is necessary of course. Under the beetle, it looks beautiful, and the length of this edeagus with a bag is about 3 cm with a tail, when you make hundreds of bags of carabuses in appearance, this is the only way to quickly and clearly compare everything.

the second option - I don't want to fill it - because "air" can always be blown out, and filled ones like toothpaste or something else-there is already a final product.

the second option is that I don't want to fill it - since "air" ones can always be blown out, and filled ones like toothpaste or something else - there is already a final product.

Perhaps I should also change the ones I did? Perhaps the second time will be dealt with?

I did it so often and it turned out very well - but the main thing is not to wet it in any alkalis - then you can tear it

So I'll try to cheat. If it breaks, then the fate is like this

An amateur question. Why boil the drug, if you can just leave it overnight in the alkali and by morning, as a rule, everything will already be quite limp and ready for viewing and analysis?

An amateur question. Why boil the drug, if you can just leave it overnight in the alkali and by morning, as a rule, everything will already be quite limp and ready for viewing and analysis?

And if you don't have time to wait until morning? and need it fast?

An amateur question. Why boil the drug, if you can just leave it overnight in the alkali and by morning, as a rule, everything will already be quite limp and ready for viewing and analysis?

This is not always the case.

An amateur question. Why boil the drug, if you can just leave it overnight in the alkali and by morning, as a rule, everything will already be quite limp and ready for viewing and analysis?

I've been doing this for years.

An amateur question. Why boil the drug, if you can just leave it overnight in the alkali and by morning, as a rule, everything will already be quite limp and ready for viewing and analysis?

So that if you're already working on this case, you can finish it quickly.

An amateur question. Why boil the drug, if you can just leave it overnight in the alkali and by morning, as a rule, everything will already be quite limp and ready for viewing and analysis?

You need to go to work in the morning

I prefer to completely clean the genitals of females with different sawyers, including removing spermatophores from the bursa.

In this case, the ductus bursa is very thin and narrow, so the spermatophore is removed through an artificially made screwdriver. In one place, dark formations and growths appeared on the bursa wall as a result of some disease. When you try to remove them, you get a hole through which you can clear the contents of the bursa.

We hook the spermatophore and partially pull it out on the rifle




We pull the spermatophore from the appendix bursa with tweezers




Pulled out



Now it is necessary to pull the remaining part out of Bursa.
We attach the tip of the bursa with a needle and pull the spermatophore with tweezers



The expanded tip of the spermatophore is barely wide in the narrow tube of the bursa



and it ends



Remove the remaining spermatophore with a needle



we wash the bursa with water using a syringe



It is necessary to inflate the bursa to restore volume. This is followed by washing with alcohol and staining with eosin.



In this form, the volume structure will be stored in the euparal.

This post was edited by barko - 04.10.2012 18: 02

is it hard to read the topic first? everything is there. there are detailed instructions on the ZIN website. open the permutation index on the letter " E " and find the word endophalus....

I prefer to completely clean the genitals of females with different sawyers, including removing spermatophores from the bursa.

Very timely post. I just wanted to ask a question about this, because I don't know what to do with these spermatophores. Oleg, is it always possible to extract them? They often twist and take on a complex shape in the bursa. I just messed up one bursa trying to get a spermatophore like this. Now, if only some selective solvent were injected into the bursa, so that it would dissolve everything inside, and not damage the walls.

Very timely post. I just wanted to ask a question about this, because I don't know what to do with these spermatophores. Oleg, is it always possible to extract them? They often twist and take on a complex shape in the bursa. I just messed up one bursa trying to get a spermatophore like this. Now, if only some selective solvent were injected into the bursa, so that it would dissolve everything inside, and not damage the walls.

I always remove spermatophores. However, it is not always possible to do this without losses.
Female Cardepia can usually be easily cleaned through the ductus bursa. I use tweezers, a needle with a hook and a syringe.











Well, if the antrum and ductus bursa are wide enough for the passage of spermatophores or their parts, if not, then something has to be sacrificed. In such cases, I make a hole in the wall of the bursa body and clean everything through it.
For example, a female Bryophila with an incision on her bursa, which I made specifically for cleaning her cavity.



In Polymixis, the antrum is wide, but the ductus bursa has a bend through which nothing can be dragged. Females of this genus have thin bursa walls compared to other parts, so holes are often formed. Through such a hole, everything can be cleaned.

Very timely post. I just wanted to ask a question about this, because I don't know what to do with these spermatophores. Oleg, is it always possible to extract them? They often twist and take on a complex shape in the bursa. I just messed up one bursa trying to get a spermatophore like this. Now, if only some selective solvent were injected into the bursa, so that it would dissolve everything inside, and not damage the walls.

Tell me which butterfly you're working with and I'll try to give you some specific advice.

Tell me which butterfly you're working with and I'll try to give you some specific advice.

With Hadena caesia/clara/melanochroa females.
And since we're talking about them, I'll ask you a question right away. Do you normally distinguish females of these species by their genitals? I can't follow the pictures from the Hacker's revision. Well, I don't see any stable differences there. Can you tell me something, especially about caesia/clara?

With Hadena caesia/clara/melanochroa females.
And since we're talking about them, I'll ask you a question right away. Do you normally distinguish females of these species by their genitals? I can't follow the pictures from the Hacker's revision. Well, I don't see any stable differences there. Can you tell me something, especially about caesia/clara?

I'm not into hadenes. And what is the difficulty? Post photos of the female's genitals and let's try to identify them together.

I don't get carried away either, but I had to. Where is it better to put it, in the definition, or images-Hadeninae? The difficulty is that it is difficult to photograph them properly-the bags are full of spermatophores, and I haven't got the hang of getting them out yet, I tried it yesterday - I ruined one. I'll try again today.

I don't get carried away either, but I had to. Where is it better to put it, in the definition, or images-Hadeninae? The difficulty is that it is difficult to photograph them properly-the bags are full of spermatophores, and I haven't got the hang of getting them out yet, I tried it yesterday - I ruined one. I'll try again today.

It is better to post in the topic Hadeninae.

... Can you post an eosin staining technique in the topic on genital dissection? They promised to get it for me here. Can eosin-stained preparations be stored in glycerol?

Everything is extremely simple - eosin crystals are added to cheesy methyl alcohol. The genitals are placed in the solution (from a few minutes to several days). I don't use glycerin to store my genitals.

and you can learn more: the amount of alcohol and eosin, which determines the exposure time and td

or you can learn more: the amount of alcohol and eosin, what determines the exposure time, and so on

all by eye

by the way, you don't have to use dangerous methanol! in ethanol, everything is exactly the same.

by the way, you don't have to use dangerous methanol! in ethanol, everything is exactly the same.

Ethanol is not suitable for euparal preparations.

Ethanol is not suitable for euparal preparations.

Why?

Everything is extremely simple - eosin crystals are added to cheesy methyl alcohol. The genitals are placed in the solution (from a few minutes to several days). I don't use glycerin to store my genitals.

There is another method. The ready-made mixture (azur - eosin according to Romanovsky) is diluted with distilled water, the genitals are placed in it for 3 to 30 minutes, depending on the degree of staining you want to achieve. In the attach, an example is the genitals of a female Alucita kosterini, the staining time is approximately 3.5 minutes.

Wash, of course, from excess paint with alcohol-methanol or ethanol, it does not matter. I generally use isopropanol - smelly and volatile, but it cleans very quickly and well.

[attachmentid()=158895]

I read the topic, saw a lot of interesting things, well, with large ones, of course, there are fewer problems than with small ones, so the question arose - what is approximately the limit (of course lower) of the size of the aedeagus beetle so that it could be inflated?

I read the topic, saw a lot of interesting things, well, with large ones, of course, there are fewer problems than with small ones, so the question arose - what is approximately the limit (of course lower) of the size of the aedeagus beetle so that it could be inflated?

there is no limit to perfection

I read the topic, saw a lot of interesting things, well, with large ones, of course, there are fewer problems than with small ones, so the question arose - what is approximately the limit (of course lower) of the size of the aedeagus beetle so that it could be inflated?

Damn it! I missed the citation and went straight to the "flower"

the lower limit is limited only by the thickness of the capillary drawn out of the glass tube. but if you have access to such micro-tools as, for example, take DNA from a cell, then you can inflate the wing
in general, someone wrote here that they were blowing bembidionov, I think

This post was edited by AGG - 12/21/2012 15: 51

yes, I have already read about capillaries, but this is a technical limit, and how to fix it? you can certainly try to work not on weight, but on a substrate - you can stick it firmly there... I already looked at it - bembidone is too large - 5 mm, although I don't see any data on edeagus yet. maybe less than 1 mm...
P.S. My flower is "real"

This post was edited by Chromocenter - 12/21/2012 16: 06

in the sense of "attaching" to the capillary? or later? if to the capillary, then it is glued, and after too. naturally, this is not done "on the knee". well, if 5 mm is a large beetle, then I'm sorry, especially since their ratio to body size significantly loses to leaf beetles. in some skrytoglavov edeagus in half the size of a beetle, I dissect all alticins, but I have never heard of the use of signs of an inverted bag in this group, probably at this stage there are enough "external" differences. who knows, now you will inflate a dozen cruciferous fleas, and there 5-6 species thank God have not reached this yet, although who knows, it seems that Kasatkin wrote about the importance of the structure of the endophalus in the taxonomy of barbels, which until recently simply no one blew...

This post was edited by AGG - 12/21/2012 17: 23

if to the capillary, then it is glued, and after too.

what can be glued to it in the best case with Limnebius has 100 microns, and in others it is good if 50 ... how to attach a capillary to tacomo and not block its channel inadvertently?..

I have never heard about the use of signs of an inverted bag in this group, probably at this stage there are enough "external" differences.

That's not what I want... I need it to assess the degree of difference in sexual characteristics. so itayama thought : if the aedeagus is so different, and there are at least no hard parts in the sexual apparatus of females, I wonder what happened to the endophalos? since they are small, they are often quite transparent and the differences in internal structures are clearly visible, but this is too small

  
... no hard parts in the sexual apparatus of females are at least not noticeable, ...

but what about spermateka!? very strongly sclerotized and distinct in appearance and is a good species-specific prio

Yes, the spermateca is visible in at least some species, although it is not used in this group. true, it can not be said that it is very strongly sclerotized... Next year, I will try to look at others who were dissected, but did not see. .. is aedeagus part of it, too? well, maybe endofalus...

naturally, he is not included in it. honestly, I lost the essence of the discussion what is the actual question

Yes, the spermateca is visible in at least some species, although it is not used in this group. true, it can not be said that it is very strongly sclerotized... Next year, I will try to look at others who were dissected, but did not see. .. is aedeagus part of it, too? well, maybe endofalus...

Edeagus with endophallus - in males, and spermateca - in females

"enters" meant in the process of mating, penetrates, and not in the anatomical sense into the composition of the organ... and spermateca is clear that in females, the question is that those structures that penetrate the genital tract of females in theory should evolve faster (assuming the prevalence of sexual selection, of course) than those that perform purely "mechanical" functions. that is, in Limnebius, the basal oedeagus lobe is almost the same for everyone, while the middle and apical parts differ simply monstrously