Preparation of genitals

I hadn't really thought about it before. Can you tell me how to determine what plastic the tube is made of? I didn't find any labels on the packaging about this. As for the difficulty of sealing, the photo is rather not a very successful sample, which was made in a hurry. If you carefully heat the tube near a low flame (for example, an alcohol lamp or a candle), it melts normally and does not burn, a flat tail is easily formed with tweezers.
The question of the reliability of gluing also bothered me, I used other adhesives before. Sometimes the tubes fell off. I have been using cyanoacrylate for 3 years, during this time there were no problems. Perhaps gluing is really worth giving up. Next time I'll try to make longer ponytails and pin the tubes through them.
My plasticine plugs do not claim to be leak-proof. They may lose their properties over time. I'll be able to tell you about it in 20 years. One colleague suggested using wax instead of plasticine, but I haven't had time to try it yet. Probably, it is possible to plug both sides with plasticine, why not. I just do what I'm used to. I also saw the option of storing drugs in such tubes, sealed on one side and open on the other. But in this case, the glycerin will dry faster.

I used various containers for genitals-both factory-made and self-made, but perhaps the most convenient and neat containers are made from PVC tubes. Unfortunately, there are no photos now, the idea is not mine, the author does not want to write on the forum. Brief instructions for their manufacture and use are provided in the attached theses.

File/s:



 ______2016____________________________________________________________________________________________________________________________________________.pdf

size: 249.48 k
number of downloads: 337

I used various containers for genitals-both factory-made and self-made, but perhaps the most convenient and neat containers are made from PVC tubes.

Unfortunately, the author did not specify exactly what "disadvantages" of cocktail tube containers do not suit him.

Going back to the size of the material. I made a selection here from Parabapta, Inurois, Aporoptrina and two moles (I will be grateful if the respected Kotbegemot can tell me who they are):



And this is their personal belongings on the same scale:



Interestingly, the moth, despite its tiny size, the genitals are quite large and it is quite easy to process them.
Photos can be enlarged.

This post was edited by Bianor - 02.11.2016 11: 06

Going back to the size of the material. I made a selection here from Parabapta, Inurois, Aporoptrina and two moles (I would be grateful if the respected Kotbegemot could tell me who they are):
And these are their personal items on the same scale:
Interestingly, the moth, despite its tiny size, the genitals are quite large and it is quite easy to process them.
Photos can be enlarged.

A new genus for the region is Damophila (this is a brilliant Coleophoridae), it looks like alcyonipennella, and maybe something more interesting.
05.08.2015, Amur region, Blagoveshchensk ?

call - is it a collector or a collection?
Who collected it ?

Thank you so much for the definition! It would be necessary to somehow send the material to the specialists, but all the opportunities will not turn up. Here, already he began to cook.

Yes, "call" is a collector, collector. Somehow I got used to it already. All these leg and det things hurt your eyes, especially when the whole geography and surname are in Russian.

That's right, the label should be in the same language. Only I write: Sobr.

That's right, the label should be in the same language. Only I write: Sobr.

Exactly, on one. And preferably on a site that is available not only here, but also somewhere else. What the hell is not joking... I once had to translate labels written in Georgian "vermicelli" for a colleague from Canada.

Who would prefer it? Someone who writes or who will then study hypothetically abroad
The one who writes is easier in their native language, and the one who will study will already have to work hard, otherwise everything is ready

Who would prefer it? Someone who writes or who will then study hypothetically abroad
It is easier for those who write in their native language, and those who will study will already have to work hard, otherwise everything is ready.

Gee-gee... The Canadian mentioned above tried to "translate" the labels from Russian on his own. It's even scary to remember how he "translated" the name of the reserve "Cedar Pad".
I had to explain to my colleague what a cedar pine is and what the word "pad" means in the east of Russia. And that no cedars fell anywhere.

PS By the way, with the current availability of devices and programs (GoogleEarth, for example), it seems very useful to insert the coordinates of the collection point in the label.

PS By the way, with the current availability of devices and programs (GoogleEarth, for example), it seems very useful to insert the coordinates of the collection point in the label.

Recently, I began to write on the back of labels for alcohol material, although this is not according to canon. And I add "WGS84" or "Pulkovo42" depending on which coordinate system the point is taken in.

That's right, the label should be in the same language. Only I write: Sobr.

everything is correct, in one language-English.
After all, you probably would not be fun to process material for the monograph from everywhere, all sorts of" dead doctors", including Korean and so on with a reprint of Oksana and other aboriginal specialists.You can also decipher idiotic abbreviations (aha, Google them at least in Hindi to find out what they are about,or filter out what was meant from the commercial slag of search results for abbreviations)...

and JPS - not in a bastard form-with minutes and up to decimal fractions of seconds...,
but in degrees with decimal fractions (x. 5795°N y. 3377°E) (you can get it by switching the type of coordinate display in Google-Es, and putting labels from where it is more convenient to copy) - and shorter on the label it turns out without loss of accuracy, and the simplicity of typing in software, and it can also mean the possibility of enlarging the font when reducing the size of the"poster".

well, the profane don't care - "any opinion has the right to exist"tm - in the era of postmodernity and baboobraznogo infantilism.

This post was edited by CosMosk - 08.12.2016 19: 19

Who would prefer it? Someone who writes or who will then study hypothetically abroad
It is easier for those who write in their native language, and those who will study will already have to work hard, otherwise everything is ready.

"we will act even more radically" - so that ungrateful enemies do not get anything interesting
- we will not collect anything smaller than 2 cm at all. And to sell May beetles is not cheaper than 5 pupaars.
And we will ask you to upload the collection with us to the crematorium-according to our native tradition.

This post was edited by CosMosk - 08.12.2016 19: 05

CosMosk, first of all, you are too emotional, and secondly, there is a corresponding topic for discussing labels:
http://molbiol.ru/forums/index.php?showtopic=70348&st=1000

For the youngest and laziest:

http://bianor.livejournal.com/39880.html

http://bianor.livejournal.com/40108.html

http://bianor.livejournal.com/40200.html

I used various containers for genitals-both factory-made and self-made, but perhaps the most convenient and neat containers are made from PVC tubes. Unfortunately, there are no photos now, the idea is not mine, the author does not want to write on the forum. Brief instructions for their manufacture and use are provided in the attached theses.

An article has been published devoted, among other things, to the manufacture of containers for the genitals of small and medium-sized insects. I've been using them myself for a long time - very convenient, neat and reliable, no comparison with Eppendorfs. In the same containers, it is convenient to constantly store small insects that are constantly stored in alcohol or alcohol-glycerin (Strepsiptera, Coniopterigidae, Embioptera, etc.).

File/s:



 ______2016____________________________________________________________________________________________________________________________________________.pdf

size: 782.57 k
number of downloads: 331

An article has been published devoted, among other things, to the manufacture of containers for the genitals of small and medium-sized insects. I've been using them myself for a long time - very convenient, neat and reliable, no comparison with Eppendorfs. In the same containers, it is convenient to constantly store small insects that are constantly stored in alcohol or alcohol-glycerin (Strepsiptera, Coniopterigidae, Embioptera, etc.).

This" wonderful " method was used a hundred years ago by many Soviet entomologists, but there is nothing new in it. In my collection there are many such preparations made from cocktail pipes. The method has one, but significant, serious drawback: the tube has to be cut when you need to get the genitals, and this is not very easy and can lead to deformations of both the tube and the micro-product.

I don't know why you don't like Eppendorfs so much. Personally, this is a godsend for me: the drug is very easy to extract into the light of day when necessary, and the storage itself is not destroyed.

This" wonderful " method was used a hundred years ago by many Soviet entomologists, but there is nothing new in it. In my collection there are many such preparations made from cocktail pipes. The method has one, but significant, serious drawback: the tube has to be cut when you need to get the genitals, and this is not very easy and can lead to deformations of both the tube and the micro-product.

I don't know why you don't like Eppendorfs so much. Personally, this is a godsend for me: the drug is very easy to extract into the light of day when necessary, and the storage itself is not destroyed.

No one says that this is "know-how". What is new here is the material for the container, and the rarely used method of closing the tube. If you have read the article, it shows how to open such a container and remove the genitals with a dissecting needle with a hook. The container is not destroyed. About the disadvantages of Eppendorfs is described in the article.

If it's not a secret, what kind of magazine accepts such articles?

Aren't you a bit blind?"

No one says that this is "know-how".

This is the Stone Age, full of pill blisters and honey. This topic has been discussed for a long time.

This is the Stone Age, full of pill blisters and honey. This topic has been discussed for a long time.
Invented cons. Especially about the fact that they open themselves or fly off the pins.

No one imposes anything on anyone!
Without trying it, it's hard to evaluate. And I tried Eppendorfs - it's cumbersome and inconvenient.

And what's wrong with such people?
http://www.omnesartes.com/262-thickbox_leo...le-da-16-mm.jpg
http://www.omnesartes.com/263-thickbox_leo...micro-vials.jpg

And what's wrong with such people?
http://www.omnesartes.com/262-thickbox_leo...le-da-16-mm.jpg
http://www.omnesartes.com/263-thickbox_leo...micro-vials.jpg

I didn't like them because it's hard to close the lid. Ependorphs benefit significantly in this regard

This post was edited by AGG-02.04.2017 17: 34

And what's wrong with such people?
http://www.omnesartes.com/262-thickbox_leo...le-da-16-mm.jpg
http://www.omnesartes.com/263-thickbox_leo...micro-vials.jpg

The article states that compressed air tends to push the plug out.

No more bulky than blisters or tubes.

I do not claim that the proposed method is universal - read the title of the article. Maybe large butterflies have large genitals and won't fit properly in a tube with an internal diameter of 3 mm, not my subject. But for small and medium-sized insects, the genitals of which must be stored in alcohols (glycerin, etc.), this method is very good. And no one bothers to take a larger diameter tube. And small delicate insects can be stored in them on pins.

Well, if Eupithecia is "large butterflies"...

Here is another such question. Has anyone tried painting their genitals with methylene blue? How does it work for chitin?

I am looking for a suitable transparent substrate for sticking minutiae when straightening the genitals. Something like a dense gel that does not interact with glycerin. I used isolon, but it is opaque, and gelatin spoils quickly.
Who can recommend what?

I am looking for a suitable transparent substrate for sticking minutiae when straightening the genitals. Something like a dense gel that does not interact with glycerin. I used isolon, but it is opaque, and gelatin spoils quickly.
Who can recommend what?

A thin layer of wax/paraffin?

It's me)

Who can tell you where to buy koh10 % lye for genital preparation?

A thin layer of wax/paraffin?

Poor balance between thickness and transparency. Well, either my wax is bad - in order for the pins to hold up normally, you have to make a layer of this thickness when the light is already poorly transmitted.

The article states that compressed air tends to push the plug out.

This is how to compress air :D

I used both test tubes with a silicone stopper, and test tubes with a native (plastic) one. I will say that the native cork is better, but it becomes a problem to put the test tube on the pin normally. I solved this problem by making a hole in the test tube lid on the side and inserting a pin into this hole. Very secure and can be attached to any pin. Personally, I hang on all numbers starting from 000 and ending with 3. And no problems )))

Who can tell you where to buy koh10 % lye for genital preparation?

In the store of chemical reagents, you can buy it in dry form and dissolve it to get a 10% solution

In the chemical reagent store, you can store it in dry form and dissolve it to get a 10% solution

is it even harmful to the eyes when you cook it?And in case of contact with the respiratory tract ?

is it even harmful to the eyes when you cook it?And in case of contact with the respiratory tract ?

You will not cook in a saucepan Lye is not volatile, so only possible splashes are dangerous. And so, of course, harmful, including in contact with the skin.. Only volumes use small sovsem

And why cook at all?

I have been using the cold method for several years now - in my opinion, there is nothing better. No fumes, no boiling (those who have cooked in test tubes will understand me - sometimes the alkali "shoots" when boiling, and you have to search for a long time where the belly has flown). We break as many bellies as we need, file them in numbered Eppendorf tubes (the label with the number is also under the copy) - and that's it. I wait a day (3% lye solution) for small and medium-sized butterflies; large butterflies settle for two days. After that, I'll dissect it. If nedomatserirovalo-throw back in the test tube and wait another day. I charge from 5 to 20 bellies for each day, depending on the expected load for the next day. 5 preparations I process 1 hour on average (I prepare, remove, pack). Determination and everything else goes later on ready-made preparations - the photo of the butterfly is also attached to the folder with genital "sections". Everything turns out very quickly and productively after certain training sessions=). I conduct the preparation with very thin needles (in the photo - for example ,a "standard" preparation needle and those with which I use the genitals of chickens), but sometimes their thickness does not satisfy me. But to find anything thinner and strong enough, alas, has not yet been possible. Although they made me titanium needles, 3 times thinner than the ones I use now. If I get it, I'll unsubscribe.

People, and by any chance there is no video of some kind, which shows the procedure for extracting and preparing the drug, in small coleoptera? well, or just in coleoptera.