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Preparation of genitals

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29.03.2015 11:30, barko

... What should I do if I need an image of the 8th sternite, like Eupithecia or scopula? ...

genitals separately, abdomen separately. All segments are visible.
picture: op0192m_edited_1.jpg

Likes: 1

29.03.2015 11:48, barko

... What should I do with the tympanic organ? ...

I try to leave it in the folded state. It doesn't always work out.
picture: 002_edited_12.jpg

29.03.2015 12:01, barko

And ostium?

Ostium is just a hole, a hole.

29.03.2015 18:46, Zunimassa

Hi.

How do large plastic sawyers get it more correctly?
and whether it is necessary to separate and glue or it is possible just to stick out?
I'd never done it before, and now I had to.

is everything written correctly here?

http://www.vietnaminsect.com/2014/07/how-t...-of-scarab.html

29.03.2015 20:57, kovyl

http://stankorb.com/articles.php?article_id=12

This I do not

have In stock ordinary MBS-10.
I also wanted to ask barko: Oleg, how do you take photos of drugs?

This post was edited by kovyl - 29.03.2015 21: 00

29.03.2015 21:33, barko

This I do not have In stock ordinary MBS-10.
I also wanted to ask barko: Oleg, how do you take photos of drugs?

Moticam 2500

30.03.2015 12:51, Bianor

Ostium is just a hole, a hole.

If it is not difficult, show examples of good preparations of Scopula females (something like umbelaria or nigropunctata, with powerful multi-layered appendages) and Abraxas. To make it clear what to strive for.

And one more question, as part of the educational program. Saving the belly is understandable, but for what purposes should I take a photo of it? The tympanic organ is not included in the definition, the other structure is almost identical, only the 8th sternite remains, and even then not in all groups.

PS
Also interested in what dye gives such a color and in general, is it able to color chitin?

This post was edited by Bianor-30.03.2015 13: 26

30.03.2015 17:55, Konung

Also interested in what dye gives such a color and in general, is it able to color chitin?

the dye appears to be eosin

30.03.2015 19:12, Bianor

the dye appears to be eosin

Eosin gives pink or orange tints, depending on the time of painting and other factors. In addition, eosin does not stain chitin, it is stuffed into the cavities. Eosin stains the cavities of the valvae, the contents of the aedeagus or bursa, can even stain the internal cavities of the setae and they become clearly visible, but if the fragment consists of one pleura, without cavities, eosin will not paint it at all. Rather, it will stick a little on top, but after you tinker with it on the slide, there will be no trace of the painting. Something else was used here.

30.03.2015 21:58, barko

I use only eosin, which perfectly colors chitin, but the membranes are slightly colored by it.

30.03.2015 22:00, kovyl

Moticam 2500

Cool!
Then another question, I apologize that it's not quite in the subject. Recently (several years), articles with descriptions of taxa, well, or in others, instead of drawings, there are more and more photos. This is due to: the availability of digital technology, laziness to draw, or a photo is better than a drawing? Something else?

30.03.2015 22:05, Bianor

I only use eosin

Are green preparations also colored with eosin? And how do you prepare the solution?

30.03.2015 22:18, barko

Cool!
Then another question, I apologize that it's not quite in the subject. Recently (several years), articles with descriptions of taxa, well, or in others, instead of drawings, there are more and more photos. This is due to: the availability of digital technology, laziness to draw, or a photo is better than a drawing? Something else?

Taking a digital photo is not a very big problem these days. Passable photos are obtained even if you simply attach a camera with a small lens to the eyepiece of a microscope. They can easily be used in a publication (I already wrote about this in some topic). My opinion is that we should avoid drawing genitalia when describing new taxa. You can certainly give drawings, but only in addition to photos.

30.03.2015 22:25, barko

Are green preparations also colored with eosin? And how do you prepare the solution?

they are not green : )) these are the features of the camera.
picture: 13_x_18_____2__copy_edited_1.jpg

This post was edited by barko - 30.03.2015 22: 26

30.03.2015 22:30, barko

Are green preparations also colored with eosin? And how do you prepare the solution?

99.5% methanol + eosin

This post was edited by barko - 03/31/2015 12: 39

31.03.2015 0:52, kovyl

... My opinion is that we should avoid drawing genitalia when describing new taxa. You can certainly give drawings, but only in addition to photos.

And why?

31.03.2015 12:42, barko

And why?

A drawing can't convey the features of a building in the same way as a photo.

Likes: 2

31.03.2015 22:33, kovyl

A drawing can't convey the features of a building in the same way as a photo.

I don't quite agree. Drawing is different from drawing, just like photos. Both are successful and not so successful.

31.03.2015 23:51, Barnaba

Imho, drawing always introduces an element of subjectivity, some (more important from the point of view of this researcher) structures are shown in more detail, sometimes with more or less conscious or unconscious distortion of proportions/exaggeration of differences, while others are insufficiently detailed or omitted altogether. This creates additional difficulties for subsequent researchers, especially if there is no direct access to the drugs/specimens. Therefore, photos are much better, and, taking into account a number of technical corrections, can often be processed meristically.

07.04.2015 23:13, barko

If it is not difficult, show examples of good preparations of Scopula females (something like umbelaria or nigropunctata, with powerful multi-layered appendages) and Abraxas.

Preparation of female Abraxas sylvata.

Likes: 2

07.04.2015 23:16, barko

What is wrong with these drugs?

In the female, a segment of the abdomen is not separated, which is why part of the genital apparatus is not visible.
picture: 0_99b17_c927a6b9_XL.jpg

Likes: 2

23.04.2015 0:21, kovyl

I tried to take pictures of the drug, but the result was completely uninspired...
I'll ask the specialists - what am I doing wrong?
Zeiss binocular, Canon A640 camera, shutter speed 1/100, aperture 5.6

Pictures:

IMG_6991.jpg — (1.86 mb)

15.10.2015 15:21, Copris

Greetings friends ! Just started to understand this, here is the question, is it possible to extract edeagus from dry beetles, pre-steaming them, will it be correct? will it affect the eversion of the endophallus?

15.10.2015 16:16, AGG

extract edeagus from a normally soaked beetle is no more difficult than from a fresh one just with a needle, but inflate it-it depends on how the beetle was caught and mortified, formalin, gasoline, acetone - full pitchforks

Likes: 1

15.10.2015 16:24, Copris

The beetles are starved with ammonia solution, isn't that a bad idea? and what is better in general?

15.10.2015 16:29, AGG

what kind of beetles are we talking about? large animals are starved with an injection of ammonia. everything gets out of these quite easily, but you need to take into account the size of WHAT you will extract

, I advise you to slightly expand the anal opening by cutting the last ventrit from the sides

This post was edited by AGG - 10/15/2015 16: 34

Likes: 1

15.10.2015 17:45, Copris

well, in general, the case is behind geotrups, crunches, another question is when I will extract the edeagus, what is the right thing to do with it, it is likely that it will also need to be steamed separately, what is the best way? I apologize for the banal questions) I just want to study everything from and to

15.10.2015 18:47, Zlopastnyi Brandashmyg

I'm not at all an expert in beetle dissection, but I can give you a general tip: take the material that you don't mind spoiling and fill your hand.

Likes: 2

15.10.2015 20:09, ИНО

17.10.2015 23:45, Troglodit

Lots of questions. What is the difference between Canadian balsam and euparal? How long-lasting are the drugs in the latter? The composition of this resin is not clear.
Another question about eosin. There is eosin of potassium and sodium, all the same? And, I didn't understand from the discussion above, only methanol or ethanol is also suitable?
And-last-advise where to buy all this cheap

.

This post was edited by Troglodit - 10/18/2015 00: 00

18.10.2015 20:44, greengrocery

Maybe there are some improvised materials for staining chitin? Iodine? Potassium permanganate?

I repeat Bianor's question and partially answer it myself.
An English colleague successfully used the dye chlorazole black E to stain chitinous membranes (poison gland reservoir, metasomal membranes and glandsThis dye seems to be used by mycologists, but domestic entomologists do not seem to practice it. And it's not easy to find it. Therefore, alternative methods of membrane staining are still interesting.

18.10.2015 20:54, Vlad Proklov

Chlorazole is commonly used in the UK. Preparations are obtained in blue color.

Likes: 1

18.10.2015 22:24, lepidopterolog

Membranes are well stained with Evans blue. Just a few seconds is enough.

Likes: 2

18.10.2015 22:29, lepidopterolog

Lots of questions. What is the difference between Canadian balsam and euparal? How long-lasting are the drugs in the latter? The composition of this resin is not clear.
Another question about eosin. There is eosin of potassium and sodium, all the same? And, I didn't understand from the discussion above, only methanol or ethanol is also suitable?
And-last-advise where to buy all this cheap .

The difference is in the optical properties, plus the Canadian balm darkens over time. Euparal is a mixture of camsal, sandrac, eucalyptol, and paraldehyde and has a higher refractive index than Canada balsam.
I dissolve eosin in ethanol.

Likes: 1

17.01.2016 11:42, Bianor

Likes: 1

18.01.2016 13:00, rhopalocera.com

I have perfectly soluble eosin in an aqueous 70% ethanol solution. And it looks pretty good.

18.01.2016 16:00, Bianor

I have perfectly soluble eosin in an aqueous 70% ethanol solution. And it looks pretty good.

In a 70% solution, it dissolves due to water. I have 90% alcohol - eosin immediately turns to stone. If you add water, it starts to dissolve. I have not tried it in methylene, because it is impossible to get it in private. By the way, in your photo, the pleura is not painted at all.

19.01.2016 0:08, rhopalocera.com

In a 70% solution, it dissolves due to water. I have 90% alcohol - eosin immediately turns to stone. If you add water, it starts to dissolve. I have not tried it in methylene, because it is impossible to get it in private. By the way, in your photo, the pleura is not painted at all.

painted over. they are also visible =)

20.01.2016 11:23, Bianor

In my photos, you can also see it, but not because they were painted over, but because there is a cavity filled with paint between the pleura.

03.08.2016 12:01, Dracus

Colleagues, what kind of needles do you use when dissecting / straightening particularly small genitalia (for example, Microlepidoptera)?

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